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. 2018 Apr 18;7:e32660. doi: 10.7554/eLife.32660

Figure 2. TRIM21 RING dimerization is not required to catalyse ubiquitin release from E2:Ub.

Figure 2.

(A) Model of the TRIM25 RING:Ube2N:Ub complex (5EYA), showing interactions between ubiquitin and both RING monomers. (B–D) Ubiquitination activity of WT T21R and mutants of putative ubiquitin-contacting residues in the second monomer. (B) Catalysis of unanchored ubiquitin chains using Ube2N. (C) Synthesis of anchored K63-chains on TRIM21 in presence of both Ube2N/Ube2V2 and Ube2W. (D) Catalysis of ubiquitin discharge from conjugated Ube2N/Ube2V2.