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. 2018 Apr 18;7:e32660. doi: 10.7554/eLife.32660

Figure 3. The Box is an autoinhibitory domain which supresses E3 ligase activity.

Figure 3.

(A) Catalysis of Ube2N/Ube2V2 driven polyubiquitin-chain synthesis by T21 RING (R), RING-Box (RB) and MiniTRIM21 (MT). (B) T21 R or RB catalysed ubiquitin discharge from Ube2D1 or Ube2N. (C) 2 Å X-ray structure of T21 RB coloured by domain with zinc ions in cyan. (D) Superposition of T21 with T5 model 4TKP (grey), showing Ube2N and the Box are structurally competitive. (E) Surface representation of (left-to-right) T21 R, Box and Ube2N binding sites coloured by electrostatic potential from −5 kT/e (red) to + 5 kT/e (blue).