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. 2018 Apr 18;7:e32660. doi: 10.7554/eLife.32660

Figure 6. TRIM21 is phosphorylated upon immune stimulation.

Figure 6.

(A) Full-length recombinant lipoyl-T21 protein phosphorylation in vitro by IKKβ and TBK1. (B–C) Expression of TBK1 (B) or upstream adaptor MAVS (C) in 293 T cells phosphorylates and stabilises C-terminally His-tagged T21. (D) 293 T cells lacking T21 reconstituted with His-tagged T21 and challenged with poly(I:C). Increased phosphorylation of T21 detected by immunoblot with pS80 antisera. (E) As in (D), except cells infected with adenovirus 5 (AdV) in the presence of IgG and T21 immunoprecipitated 7 hr post-infection.