Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Caenorhabditis elegans) | zhp-1 | WormBase/This paper | WormBase ID: F55A12.10; WBGene00018867 | |
| Gene (Caenorhabditis elegans) | zhp-2 | WormBase/This paper | WormBase ID: D1081.9; WBGene00008387 | |
| Gene (Caenorhabditis elegans) | zhp-3 | WormBase/(Jantsch et al., 2004) PMID: 15340062 | WormBase ID: K02B12.8b; WBGene00006976 | |
| Gene (Caenorhabditis elegans) | zhp-4 | WormBase/This paper | WormBase ID: Y39B6A.16; WBGene00012678 | |
| Strain, strain background (Saccharomyces cerevisiae) | S. cerevisiae: MaV203 | Invitrogen, Thermo Fisher Scientific, Waltham, MA | Cat#11445012 | |
| Strain, strain background (Caenorhabditis elegans) | For C. elegans allele and strain information, see Supplementary file 1, Tables S2 and S4. | This paper | N/A | |
| Genetic reagent | For CRISPR/Cas9 reagents, see sequence-based reagent and peptide, recombinant protein. | This paper | N/A | |
| Antibody | Rabbit polyclonal anti-SYP-1 | (MacQueen et al., 2002) PMID: 12231631 | N/A | (1:500 IF) |
| Antibody | Rabbit polyclonal anti-SYP-2 | (Colaiácovo et al., 2003) PMID: 12967565 | N/A | (1:500 IF) |
| Antibody | Rabbit polyclonal anti-HTP-1 | (Martinez-Perez et al., 2008) PMID: 18923085 | N/A | (1:500 IF) |
| Antibody | Rabbit polyclonal anti-RAD-51 |
Millipore Sigma, St. Louis, MO |
Cat#29480002 | (1:5,000 IF) |
| Antibody | Rabbit polyclonal anti-pHIM-8/ZIMs | (Kim et al., 2015) PMID: 26506311 | N/A | (1:500 IF) |
| Antibody | Rabbit polyclonal anti-ZHP-3 | ModENCODE/SDIX; (Rog et al., 2017) PMID: 28045371 | Cat#SDQ3956 | (1:5,000 IF) |
| Antibody | Rabbit polyclonal anti-MSH-5 | ModENCODE/SDIX | Cat#SDQ2376 | (1:5,000 IF) |
| Antibody | Rabbit polyclonal anti-REC-8 | ModENCODE/SDIX | Cat#SDQ0802 | (1:5,000 IF) |
| Antibody | Rabbit polyclonal anti-COH-3 | ModENCODE/SDIX | Cat#SDQ3972 | (1:5,000 IF) |
| Antibody | Rat polyclonal anti-HIM-8 | (Phillips et al., 2005) PMID: 16360035 | N/A | (1:500 IF) |
| Antibody | Goat polyclonal anti-SYP-1 | (Harper et al., 2011) PMID: 22018922 | N/A | (1:300 IF) |
| Antibody | Chicken polyclonal anti-HTP-3 | (MacQueen et al., 2005) PMID: 16360034 | N/A | (1:500 IF) |
| Antibody | Guinea pig polyclonal anti-HTP-3 |
(MacQueen et al., 2005) PMID: 16360034 | N/A | (1:500 IF) (1:1,500 WB) |
| Antibody | Guinea pig polyclonal anti-PLK-2 |
(Harper et al., 2011) PMID: 22018922 | N/A | (1:100 IF) |
| Antibody | Rabbit polyclonal anti-V5 |
Millipore Sigma | Cat#V8137; RRID:AB_261889 | (1:250 IF) |
| Antibody | Mouse monoclonal anti-V5 |
Thermo Fisher Scientific | Cat#R960-25; RRID:AB_2556564 | (1:500 IF) (1:1,000 WB) |
| Antibody | Mouse monoclonal anti-FLAG M2 | Millipore Sigma | Cat#F1804; RRID:AB_262044 | (1:500 IF) (1:1,000 WB) |
| Antibody | Mouse monoclonal anti-HA, clone 2–2.2.14 | Thermo Fisher Scientific | Cat#26183; RRID:AB_10978021 | (1:400 IF) (1:1,000 WB) |
| Antibody | Mouse monoclonal anti-GFP |
Millipore Sigma | Cat#11814460001; RRID:AB_390913 | (1:500 IF) (1:1,000 WB) |
| Antibody | Mouse monoclonal anti-α-tubulin, clone DM1A | Millipore Sigma | Cat#05–829; RRID:AB_310035 | (1:5,000 WB) |
|
Recombinant DNA reagent |
Peft-3::AID::GFP::unc-54 3’UTR | (Zhang et al., 2015) PMID: 26552885; Addgene | pLZ29; Addgene plasmid #71719 | |
| Recombinant DNA reagent | cDNA zhp-1 | WormBase | WormBase ID: F55A12.10; WBGene00018867 | |
| Recombinant DNA reagent | cDNA zhp-2 | WormBase | WormBase ID: D1081.9; WBGene00008387 | |
| Recombinant DNA reagent | cDNA zhp-3 | WormBase | WormBase ID: K02B12.8b; WBGene00006976 | |
| Recombinant DNA reagent | cDNA zhp-4 | WormBase (codon-optimized byIntegrated DNA Technologies to facilitate gBlock synthesis) | WormBase ID: Y39B6A.16; WBGene00012678 | |
| Recombinant DNA reagent | Plasmid: pDEST32-zhp-1-V5 | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST32-zhp-2-3xFLAG | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST32-zhp-3-3xFLAG | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST32-zhp-4-HA | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST22-zhp-1-V5 | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST22-zhp-2-3xFLAG | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST22-zhp-3-3xFLAG | This paper | N/A | |
| Recombinant DNA reagent | Plasmid: pDEST22-zhp-4-HA | This paper | N/A | |
| Sequence-based reagent | FISH probe to the right arm of Chromosome V (5S rDNA) | (Dernburg et al., 1998) PMID: 9708740 | N/A | |
| Sequence-based reagent | FISH Probe to the right arm of X Chromosome | (Phillips et al., 2005) PMID: 16360035 | N/A | |
| Sequence-based reagent | CRISPR tracrRNA | Integrated DNA Technologies, Skokie, IL | Cat#1072534 | |
| Sequence-based reagent | For crRNAs, repair templates and genotyping primers, see Supplementary file 1,Table S3 | This paper | N/A | |
| Peptide, recombinant protein | S. pyogenes Cas9-NLS purified protein | QB3 MacroLab at UC Berkeley | N/A | |
| Chemical compound, drug | Auxin: 1H-Indole-3-acetic acid | Thermo Fisher Scientific, Waltham, MA | Cat#122160250; CAS:87-51-4 | |
| Chemical compound, drug | NuSieve 3:1 agarose | Lonza, Walkersville, MD | Cat#50090 | |
| Chemical compound, drug | Nuclease-Free Duplex Buffer | Integrated DNA Technologies | Cat#11-01-03-01 | |
| Commercial assay or kit | SuperSignal West Femto Maximum Sensitivity Substrate kit | Thermo Fisher Scientific | Cat #34095 | |
| Software, algorithm | SoftWorx package | Applied Precision; GE Healthcare Bio- Sciences, Pittsburgh, PA | http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences-us/29065728 | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/ | |
| Software, algorithm | Adobe Photoshop CC 2014 | Adobe Systems, San Jose, CA | http://www.adobe.com/products/photoshop.html | |
| Software, algorithm | T-COFFEE | Swiss Institute of Bioinformatics; (Notredame et al., 2000) PMID:10964570 | http://tcoffee.vital-it.ch/apps/tcoffee/index.html | |
| Software, algorithm | IBS_1.0.1 | (Liu et al., 2015) PMID: 26069263 | http://ibs.biocuckoo.org/download.php | |
| Software, algorithm | Clustal Omega | EMBL-EBI | http://www.clustal.org/omega/ | |
| Software, algorithm | Graphpad Prism | Graphpad Software, Inc., La Jolla, CA | http://www.graphpad.com/ | |
| Software, algorithm | MSG software package | (Andolfatto et al., 2011) PMID: 21233398 | https://github.com/JaneliaSciComp/msg |