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. Author manuscript; available in PMC: 2019 Mar 7.
Published in final edited form as: Wound Repair Regen. 2018 Mar 7;26(1):6–15. doi: 10.1111/wrr.12616

Figure 1. Generation of Postn-overexpressing mice.

Figure 1

(A) Structure of the transgene. Transgene contains the ubiquitous CAG promoter and the full-length periostin cDNA followed by the rabbit β-globin polyadenylation signal. Arrows indicate the positions of forward and reverse primers used for transgene specific-PCR. (B) A representative PCR genotyping result from tails of Postn-overexpressing and non-transgenic mice. Skin sections and sera were collected from naïve Postn-overexpressing lines and non-transgenic littermates. (C) qRT-PCR analysis of periostin mRNA in transgenic lines 7, 16, and 21. (D) Quantitative analysis of periostin protein in serum within lines 7, 16, and 21. The data shown are the mean ± SE of three independent experiments. Statistical analysis was performed using a one-sided, Mann-Whitney t-test; **p < 0.01 versus non-transgenic.