Figure 5. IL-15C Treatments Induce IL-10 Expression in NK Cells.

(A) WT mice (n = 3/group) were treated with IL-2C on days −7, −5, and −3 or IL-15C on days −5 and −3 relative to harvest, and splenic NK1.1⁺NKp46⁺CD3⁻ NK cells were FACS sorted. RNA-seq analysis was performed on RNA isolated from the purified NK cell populations. Genes shown were significantly differentially expressed between the two treatments with ≥2-fold change in expression. Arrowhead indicates Il10 on the heatmap.

(B and C) Il10^GFP mice were left untreated (n = 11) or treated with IL-2C (n = 8) or IL-15C (n = 11) on days −5 and −3 relative to harvest.

(B) Representative histograms of Il10^GFP expression in NK1.1⁺NKp46⁺TCRβ⁻ cells in the spleen, blood, and brain.

(C and D) The frequency (C) and total number (D) of Il10^GFP+ NK cells in the spleen, blood, and brain on days 0, 3, and 6 after PbA infection were quantified. Data are combined from four independent experiments and presented as the mean ± SEM (C and D); *p < 0.05, **p < 0.01, ****p < 0.0001 as determined by one-way ANOVA followed by Tukey’s multiple comparison test.

Related to Figures S4 and S5.