Antibodies |
NK1.1 (clone PK136) |
BioLegend |
Cat#108739; RRID: AB_2562273 |
NKp46 (clone 29A1.4) |
BioLegend |
Cat#137611; RRID: AB_10915472 |
CD3ε (clone 17A2) |
BioLegend |
Cat#100217; RRID: AB_1595597 |
TCRβ (clone H57-597) |
eBioscience |
Cat#47-5961-82; RRID: AB_1272173 |
CD4 (clone RM4-5) |
BD Biosciences |
Cat#557956; RRID: AB_396956 |
CD8α (clone 53-6.7) |
BD Biosciences |
Cat#563332; RRID: AB_2721167 |
CD44 (clone IM7) |
BioLegend |
Cat#103044; RRID: AB_2650923 |
Ly-6A (clone D7) |
eBioscience |
Cat#12-5981-81; RRID: AB_466085 |
CD43 (clone 1B11) |
BioLegend |
Cat#121217; RRID: AB_528812 |
CD11b (clone M1/70) |
BD Biosciences |
Cat#565976; RRID: AB_2721166 |
CD27 (clone LG.7F9) |
eBioscience |
Cat#17-0271-81; RRID: AB_469369 |
KLRG1 (clone 2F1) |
TONBO Biosciences |
Cat#20-5893; RRID: AB_2621607 |
IFN-γ (clone XMG1.2) |
TONBO Biosciences |
Cat#60-7311; RRID: AB_2621871 |
CD45.2 (clone 104) |
BioLegend |
Cat#109813; AB_389210 |
Ter119 |
eBioscience |
Cat#25-5921-82; RRID: AB_469661 |
CD3ε (clone 500A2) |
BD Biosciences |
Cat#553239; RRID: AB_394728 |
NK1.1 (clone PK136) |
Bio X Cell |
Cat#BE0036 |
IL-10R (clone 1B1.3A) |
Bio X Cell |
Cat#BE0050 |
Bacterial and Virus Strains |
Listeria monocytogenes-OVA |
Dr. Hao Shen, U of Pennsylvania |
(Pope et al., 2001) |
Chemicals, Peptides, and Recombinant Proteins |
Mouse recombinant IL-15 |
eBioscience |
Cat#14-8153-80 |
Mouse recombinant IL-15Rα-Fc |
R&D Systems |
Cat#551-MR |
Mouse recombinant IL-2 |
eBioscience |
Cat#14-8021-64 |
Recombinant Anti-IL-2 (S4B6) |
Bio X Cell |
Cat#BE0043-1 |
ALT-803 |
Altor Bioscience Corp |
Cat#ALT-803 |
Glutaraldehyde |
Calbiochem |
Cat#354400 |
Hoescht |
Sigma-Aldrich |
Cat#B2261 |
Dihydroethidium |
Sigma-Aldrich |
Cat#37291 |
Percoll |
GE HealthCare |
Cat#17-0891-09 |
Streptavidin-APC |
ThermoFisher |
Cat#S868 |
Streptavidin-PE |
ThermoFisher |
Cat#S21388
|
Critical Commercial Assays |
Mouse NK isolation kit II |
Miltenyi Biotec |
Cat#130-096-892 |
Foxp3 Fixation/Permeabilization Kit |
eBioscience |
Cat#00-5521-00 |
IFN-γ ELISA kit |
eBioscience |
Cat#50-173-21 |
Deposited Data |
RNA-seq |
This paper |
GEO: 109586 |
Experimental Models: Organisms/Strains |
C57BL/6 |
Charles River Laboratories |
C57BL/6NCrl |
C57BL/6-Ly5.1 |
Charles River Laboratories |
B6.SJL-PtprcaPepcb/BoyCrl |
Nr4a1GFP
|
Dr. Kristin Hogquist |
(Moran et al., 2011) |
IfngYFP
|
The Jackson Laboratory |
JAX: 017581 |
Il10GFP
|
The Jackson Laboratory |
JAX: 014530 |
Il10−/−
|
The Jackson Laboratory |
JAX: 002251 |
Il10rb−/−
|
The Jackson Laboratory |
JAX: 005027 |
Software and Algorithms |
GraphPad Prism |
GraphPad Software, Inc. |
https://www.graphpad.com/ |
TopHat |
(Kim et al., 2013) |
http://cole-trapnell-lab.github.io/projects/tophat/ |
CuffLinks |
(Trapnell et al., 2010, 2013) |
http://cole-trapnell-lab.github.io/projects/cufflinks/ |
CummRbund |
(Goff et al., 2014) |
http://compbio.mit.edu/cummeRbund/ |
Other |
Plasmodium berghei ANKA |
Dr. Susan Pierce, NIH |
N/A |