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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: J Tissue Eng Regen Med. 2018 Jan 21;12(4):e1962–e1973. doi: 10.1002/term.2627

Figure 4. Expression of developmental stage-specific markers.

Figure 4

Cells differentiated on hDLM (blue lines) or Matrigel substrates (green lines) were analyzed at the end of each stage of the differentiation protocol for markers specific to (A) pluripotency (NANOG, OCT4), (B) definitive endoderm (GATA4, SOX17, FOXA2), (C) hepatoblast (HNF4α, CK19, CK18, AFP) and (D) mature hepatocytes (HNF6, CEBDP, TAT). (Results were normalized to the highest expression level of each gene achieved under all conditions)(n = 3, error bars represent standard deviation, *p<0.05).