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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Hepatology. 2018 Mar 25;67(5):1903–1919. doi: 10.1002/hep.29652

Figure 6. IL-1β is produced earlier than CXCL10 in Pkhd1del4/del4 cholangiocytes in vivo.

Figure 6

A, B) Cholangiocytes (EpCAM+ Cells) isolation with FACS sorting from liver of 1-month-old mice. A) pro-IL-1β gene expression assessed by RT-PCR was increased whereas B) Cxcl10 gene expression was unaltered in cholangiocytes isolated from 1-month-old Pkhd1del4/del4 mice compared to WT mice of the same age. C) IL-1β (red) was expressed in cholangiocytes (K19+ cells, green) in liver specimens from 1-month-old Pkhd1del4/del4 but not from WT mice as assessed by immunofluorescence. D) CXCL10 (red) was not expressed in cholangiocytes (K19+ cells, green) either in WT or in Pkhd1del4/del4 in 1-month old mice. (*p<0.05, Students Ttest). FPC−: Fibrocystin-defective cholangiocytes. K19: Cytokeratin-19.