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. Author manuscript; available in PMC: 2018 Apr 19.
Published in final edited form as: Handb Exp Pharmacol. 2018;245:271–312. doi: 10.1007/164_2017_62

Figure 9. Confocal microscopy analysis of hA trafficking in pancreatic cells.

Figure 9

hA was incubated with cells for 24 hours, cells fixed and its trafficking and association with cellular organelles, cytosolic and nuclear proteins was analyzed by indirect immunocytochemistry. Association of hA or lack of it with lysosomes (LAMP1), mitochondria (Mitotracker-MITO), Golgi (GM130), heat shock protein (HSP70) and ubiquitin (PD41) is shown. Representative cells in which hA accumulates in LAMP2 positive perinuclear compartments and interacts with ubiquitin in cytosol and nucleus are indicated by arrows.