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. 2000 Jul;123(3):1005–1014. doi: 10.1104/pp.123.3.1005

Figure 2.

Figure 2

Restriction map of the OsTubA1 gene and construction of the chimeric plasmid between the OsTubA1 promoter and GUS gene. A, Restriction map of the OsTubA1 gene. The coding regions and introns are represented by white and black bars, respectively. The promoter (5′) and terminator (3′) regions are shown. The arrow indicates direction of transcription. B, Diagram of pGA2135 carrying upstream sequences, exon 1 and 2, intron 1 and 2, a portion of exon 3 of OsTubA1. The HindIII site of the exon 3 of the gene is fused to the GUS gene. C, Diagram of pGA2136 carrying upstream sequences and a portion of exon 1 of OsTubA1. The XhoI site of the exon 1 of the gene is fused to GUS. Tnos signifies the nopaline synthase (nos) terminator. The GUS coding region is indicated by a solid line with an arrow. Letters represent restriction enzyme sites: B, BamHI; H, HindIII; P, PstI; R, EcoRI; S, SacI; Xb, XbaI; Xh, XhoI.