Fig. 5.

Selective overexpression of TAp63 in POMC neurons reduces the body weight of male HFD-fed mice. a Schematic construction of AAV vector, AAV-FLEX-TAp63-2A-GFP. b Immunofluorescent images showing GFP (left panel), TOMATO (middle panel), and merge (right panel) in POMC neurons of POMC-Cre/Rosa26-tdTOMATO mice receiving AAV-FLEX-TAp63-2A-GFP stereotaxically injected in the ARH bilaterally. The majority of GFP-labeled neurons are TOMATO-positive; only a few double-labeled neurons are pointed by arrowheads for a better view. 3 V, 3rd ventricle; ARH, arcuate nucleus of the hypothalamus. c Changes in body weight in HFD-fed male mice. Data are presented as mean ± SEM. N = 7 or 12 in control or OE group; N = 4 in one side group. *P < 0.05 between groups and ^#P < 0.05 between the control and pomc-TAp63 OE groups at each time point in two-way ANOVA followed by post hoc Sidak tests. d Changes in the fat and lean mass in HFD-fed male mice. Data are presented as box and wiskers showing minimal, maximal, and median values with individual data points. N = 7 or 12 in control or OE group; N = 4 in one side group. *P < 0.05 in two-way ANOVA followed by post hoc Sidak tests. e Cumulative HFD intake in HFD-fed male mice. Data are presented as mean ± SEM. N = 7 or 12 in control or OE group; N = 4 in one side group. *P < 0.05 between groups and #P < 0.05 between the control and pomc-TAp63 OE groups at each time point in two-way ANOVA followed by post hoc Sidak tests. f Changes in body weight in HFD-fed female mice. Data are presented as mean ± SEM. N = 5 or 13 in control or OE group; N = 4 in one side group. g Changes in the fat and the lean mass in HFD-fed female mice. Data are presented as box and wiskers showing minimal, maximal, and median values with individual data points. N = 5 or 13 in control or OE group; N = 4 in one side group. h Cumulative HFD intake in HFD-fed female mice. Data are presented as mean ± SEM. N = 5 or 13 in control or OE group; N = 4 in one side group