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. 2018 Apr 18;9:1541. doi: 10.1038/s41467-018-04000-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — ROS and moladietz are involved in JNK persistent activity. a, b Wing discs stained 96 h after irradiation with DHE to detect ROS, a dronc⁺ control (n = 10) and b dronc⁻ mutant (n = 30). We observe DHE staining in dronc⁻ mutant disc (b, arrows) in cells that present JNK activity, labelled by the expression of UAS-GFPDbox, but not in (a) dronc⁺ control. c Wing disc of the genotype: UAS-SOD:UAS-Cat/UAS-GFPtubGal80^ts;droncⁱ²⁴/droncⁱ²⁹hh-Gal4 96 h after irradiation (n = 14) (after irradiation, larvae were raised for 24 h at 17 °C to allow ROS activation and then transferred to 29 °C for 72 h to block ROS production). We observe a reduction in the activation of JNK (puc-LacZ, red) only in the posterior compartment (GFP, green), where SOD:Cat are expressed. Wing discs of the genotypes: d (n = 9) TRE:RFP/mol-LacZ and e (n = 22) TRE:RFP/mol-LacZ;droncⁱ²⁴/droncⁱ²⁴ 96 h after irradiation where mol-LacZ expression (green) is maintained in dronc⁻ mutants (e) in cells that show JNK activity (TRE:RFP, red). No RFP or ectopic mol-LacZ is observed in (d) dronc⁺ control. f Wing disc of the genotype: UAS-molRNAi/UAS-GFPtubGal80^ts;droncⁱ²⁴/hh-Gal4droncⁱ²⁹ (n = 7) 96 h after irradiation. As in c, the mol RNAi was expressed the last 72 h after irradiation. Reduction of the JNK activity (Mmp1, red) is observed in the posterior compartment (green), but not in the anterior. Scale bars are 100 μm