Figure 10.

Effects of intracellular signaling pathway inhibitors on isotocin (Ist) inhibition of growth hormone (Gh) release. The primary cultured pituitary cells were pre-incubated for 12 h before being treated with Ist (100 nM) in the presence or absence of inhibitors Rp-cAMPS (50 µM), Go6983 (10 µM), U73122 (10 µM), or 2-APB (100 µM) for 12 h, respectively. After drug treatment, the culture medium was harvested for measurement of Gh release by competitive ELISA. Data were expressed as fold change relative to the vehicle control in the absence of inhibitors. Bars represent mean ± SEM of pooled results from four separate experiments (n = 4). *P < 0.05 between the indicated groups, ^#P < 0.05 vs. the group treated only with isotocin (one-way ANOVA followed by the turkey test).