Figure 6.

The cellular localization of immunoreactive isotocin (Ist) receptor 1 (Istr1) (green), Istr2 (green), and prolactin (Prl) (red) in the pituitary of female, intersexual, and male ricefield eels. The rabbit antiserum against Istr1 (1:500) or Istr2 (1:500) and mouse antiserum against Prl (1:800) were used as primary antisera. The secondary antibodies were Alexa Fluor 488-labeled goat anti-rabbit immunoglobulin G (H + L) for Istr1 and Istr2, and Cy3-labeled goat anti-mouse lgG (H + L) for Prl. DAPI was used to stain the nuclei blue. Sagittal sections of ricefield eel pituitaries were shown here with the rostral (anterior) to the left. (A1–F1) are higher magnification of (A–F), respectively. (G–I): HE-stained gonads of the experimental fish at female, intersexual, and male stages, respectively. Scale bar is 50 µm.