Figure 9.

Effects of isotocin (Ist) (100 nM) on prl gene expression and prolactin (Prl) contents in ricefield eel pituitary cells. The primary cultured pituitary cells were pre-incubated for 12 h before treating with Ist (100 nM) for 1, 3, 6, and 12 h. prl mRNA levels (A) were analyzed by real-time PCR, and Prl contents were examined with Western blot analysis (B) and further quantified (C) with Gel-Pro analyzer software by applying the integrate optical density, and normalized by those of Actb. Data were expressed as fold change relative to the corresponding control. Bars represent mean ± SEM of pooled results from three separate experiments (n = 3). Ctrl, vehicle control.