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. 2018 Apr 12;9:771. doi: 10.3389/fimmu.2018.00771

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Copyright © 2018 Hyvärinen, Holopainen, Skirdenko, Ruhanen, Lehenkari, Korhonen, Käkelä, Laitinen and Kerkelä.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of MSC coculture on the level of cytokines in Mreg-conditioned media. The media were analyzed for 18 cytokines and measures within the cytokine-specific detection range were included in the analysis. The values of cell culture media cytokines were log10-transformed. The variation of cytokines between Mreg-conditioned media with and without MSC coculture was analyzed by the Wilcoxon matched-pairs signed-rank test. The number of biological replicates varied from 9 to 12. Abbreviations: IL, interleukin; Mreg, regulatory macrophage; MSC, mesenchymal stromal cell.