Table 3.
Levels of lipid mediators (LMs).
| LM | Tandem mass spectrometry |
Mreg-conditioned media w/o MSC coculture |
Mreg-conditioned media w/o MSC-EVs |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Mass/charge |
Median (IQR) (ng/ml)a |
Median (IQR) (ng/ml)b |
|||||||
| Q1 | Q3 (quant) | Q3 (qual) | Mreg (n = 12) | Mreg + MSC (n = 12) | p-Valueb | Mreg (n = 8) | Mreg + MSC-EV (n = 8) | p-Valuec | |
| Arachidonic acid-derived | |||||||||
| TxB2 | 369 | 169 | 195 | 15.5 (13.8) | 18.4 (11.2) | 1.000 | 19.6 (12.8) | 12.1 (13.2) | 0.547 |
| PGE2 | 351 | 271 | 189 | 0.3 (0.9) | 4.2 (2.8) | 0.002 | 0.0 (0.6) | 0.6 (0.9) | 0.031 |
| PGD2 | 351 | 189 | 233 | 0.0 (0.0) | 0.0 (0.2) | 0.250 | ND | ND | ND |
| 15-Deoxy-Δ12,14-PGJ2 | 315 | 271 | 203 | ND | ND | ND | ND | ND | ND |
| LTB4 | 335 | 195 | 59 | ND | ND | ND | ND | ND | ND |
| LXA4 | 351 | 115 | 217 | ND | ND | ND | ND | ND | ND |
| 15-HETE | 319 | 219 | 301 | 0.5 (1.0) | 0.8 (1.0) | 0.020 | 0.6 (0.1) | 0.8 (0.8) | 0.313 |
| Eicosapentaenoic acid-derived | |||||||||
| 18-HEPE | 317 | 215 | 259 | 0.4 (0.4) | 0.4 (0.1) | 0.193 | 0.5 (0.3) | 0.4 (0.3) | 0.148 |
| Docosahexaenoic acid-derived | |||||||||
| RvD1 | 375 | 215 | 233 | ND | ND | ND | ND | ND | ND |
| RvD2 | 375 | 215 | 175 | ND | ND | ND | ND | ND | ND |
| RvD3 | 375 | 147 | 137 | ND | ND | ND | ND | ND | ND |
| 10S,17S-diHDHA | 359 | 153 | 206 | ND | ND | ND | ND | ND | ND |
| MaR1 | 359 | 177 | 250 | ND | ND | ND | ND | ND | ND |
| 17-HDHA | 343 | 245 | 201 | 0.7 (1.2) | 1.1 (1.6) | 0.009 | 1.0 (0.5) | 0.9 (0.7) | 0.945 |
| 14S-HDHA | 343 | 205 | 161 | 0.4 (0.4) | 0.4 (0.7) | 0.359 | 0.3 (0.2) | 0.4 (0.6) | 0.078 |
diHDHA, dihydroxydocosahexaenoic acid; EV, extracellular vesicle; HDHA, hydroxydocosahexaenoic acid; HEPE, hydroxyeicosapentaenoic acid; HETE, hydroxyeicosatetraenoic acid; IQR, interquartile range; LT, leukotriene; LX, lipoxin; MaR, maresin; Mreg, regulatory macrophage; MSC, mesenchymal stromal cell; ND, not detected; PG, prostaglandin; Qual, qualifier; Quant, quantifier; Rv, resolvin; Tx, thromboxane; w/o, with or without.
aThe LMs were identified using liquid chromatography–tandem mass spectrometry method with detection limit <0.2 ng/ml.
bThe statistical significance of variation between Mreg-conditioned media and Mreg-conditioned media with MSC coculture was determined using the Wilcoxon matched-pairs signed-rank test.
cThe statistical significance of variation between Mreg-conditioned media and Mreg-conditioned media with MSC-derived EVs was determined using the Wilcoxon matched-pairs signed-rank test.