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. 2018 Apr 12;9:230. doi: 10.3389/fneur.2018.00230

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Copyright © 2018 Kichev, Baburamani, Vontell, Gressens, Burkly, Thornton and Hagberg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of hypoxia–ischemia (HI) on the expression levels of TWEAK (A,C) and Fn14 (B,D) in neonatal mice. mRNA levels were measured in contralateral (Contra; white bars) and ipsilateral (Ipsi; gray bars) hemispheres of the brain 4, 24, and 72 h after the insult. The results are expressed as fold change from contralateral side. Western blot detection of TWEAK (E) and Fn14 (F) protein and densitometry (G,H) in sham-operated (Sham, white bars), ipsilateral (I, gray bars), and contralateral hemisphere (C, black bars) brain lysates 72 h after HI. Densitometry of the TWEAK and Fn14 bands was corrected to the corresponding β-actin bands. Bars represent mean + SEM; *p < 0.05; **p < 0.01; versus corresponding contralateral side [n = 6–9 (A–D) and n = 3 (E,F)].