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. 2018 Apr 12;9:451. doi: 10.3389/fimmu.2018.00451

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Copyright © 2018 Tong, Lönnblom, Yau, Nandakumar, Liang, Ge, Viljanen, Li, Bãlan, Klareskog, Chagin, Gjertsson, Kihlberg, Zhao and Holmdahl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T cell recall assay for CII and CXI. The response of HCQ3 and HCQ4 hybridoma to native (A,C) and denatured (B,D) collagens. The supernatant was collected and the IL-2 secretion was measured by cytokine enzyme-linked immunosorbent assay. Glycosylated CII259-273 peptide (GalCII259-273) and naked CII259-273 peptide (nCII259-273) were served as positive control peptides for HCQ3 and HCQ4 hybridoma, respectively. rCII, rat Collagen II; bCXI, bovine collagen XI.