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. 2018 Apr 12;9:451. doi: 10.3389/fimmu.2018.00451

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Copyright © 2018 Tong, Lönnblom, Yau, Nandakumar, Liang, Ge, Viljanen, Li, Bãlan, Klareskog, Chagin, Gjertsson, Kihlberg, Zhao and Holmdahl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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L10D9 binds to CXI, cross-react with CII due to sequence similarity. (A) Kinetics of L10D9, L5F3, and L7D8 antibody response to bCXI, rCXI, rCII, and their denatured forms. Cartilage oligo matrix protein (COMP) was served as a negative control. Bead-based multiplex immunoassays [(B), left panel] and enzyme-linked immunosorbent assays (ELISA) [(B), middle and right panel] were used for the epitope mapping of L10D9. (C) The binding of L10D9 and CIIC2 to the heterotrimeric CXI_T_D3 peptide. (D) SDS-PAGE for rCXI and rCII (left panel). Western blotting for L10D9 (middle panel) and M2139 (right panel). (E) Sequence of the peptides involved in ELISA and bead-based multiplex immunoassays.