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. 2018 Apr 12;9:560. doi: 10.3389/fmicb.2018.00560

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Copyright © 2018 Wright, Geszvain, Oldham, Luther and Tebo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mn(II), Mn(III)-citrate, and Mn(III)-DFOB oxidation over time by (A) P. hunanensis GSL-007, (B) Pseudomonas sp. GSL-010, (C) P. putida GB-1 alongside associated mutants—(D) P. putida KG_mnxG (producing only MnxG), (E) P. putida KG_mcoA (producing only McoA) and (F) P. putida KG_mopA (producing only MopA)—as measured by Mn(III,IV) oxide formation. Mn(II) was used as a positive control with (G) sterile medium and (H) P. putida TKO (devoid of any Mn oxidase genes) serving as negative controls. Grown in minimal media at 30°C containing 100 μM of relevant Mn compound. Relative growth between bacterial isolates was normalized by OD₆₀₀ (results not shown).