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. 2018 Apr 12;9:362. doi: 10.3389/fphys.2018.00362

Figure 2.

Figure 2

Influence of single and double E8Q and E12Q mutations on (gj, eff/gj, ss)-pHi dependence modulated by [Mg2+]p. (A) For Cx36*E8Q-EGFP the effect of acidification is modulated by high [Mg2+]p, while alkalization—by low [Mg2+]p. (B) The increase of [Mg2+]p shifts peaks of Cx36*E8Q-EGFP (gj, eff/gj, init)-pHi curves to the left with the maximal gj, eff/gj, init values at pHi ~6.9–7.4 for 0.01 and 1 mM [Mg2+]p, and at pHi ~6.7–7.2 for 5 mM [Mg2+]p. (C) The decrease of gj, eff/gj, ss of Cx36*E12Q-EGFP during acidification depends on [Mg2+]p. (D) The peaks of (gj, eff/gj, init)-pHi dependencies shift to the left with the maximal gj, eff/gj, init values at pHi ~7.1–7.6 for 0.01 mM [Mg2+]p, and at pHi ~6.7–7.3 for 1 and 5 mM [Mg2+]p. (E) Double mutation causes decrease of gj, eff/gj, ss at high [Mg2+]p. (F) The peaks of (gj, eff/gj, init)-pHi dependencies are the same at 0.01 and 1 mM [Mg2+]p, and only slightly shift to the left with the maximal gj, eff/gj, init values at pHi ~6.9–7.3 for 5 mM [Mg2+]p.