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. 2018 Apr 12;9:362. doi: 10.3389/fphys.2018.00362

Figure 3.

Figure 3

Comparison between gj response of Cx36-EGFP, Cx36*E8Q-EGFP, Cx36*E12Q-EGFP and Cx36*E8Q-E12Q-EGFP to [Mg2+]p and pHi. (A) The increase of gj, ss/gj, init at 0.01 mM [Mg2+]p was observed for Cx36-EGFP (1.37 ± 0.13), E12Q (1.16 ± 0.08), and E8Q-E12Q (1.23 ± 0.16), while a small decrease was obtained for E8Q (0.97 ± 0.08). (B) The gj, ss/gj, init values at 1 mM [Mg2+]p were 1.09±0.08, 1.00 ± 0.05, 0.98 ± 0.04, and 1.13 ± 0.04 for Cx36-EGFP, E8Q, E12Q, and E8Q-E12Q, respectively. (C) The decrease of gj, ss/gj, init to 0.46 ± 0.09, 0.32 ± 0.06, and 0.40 ± 0.07 were obtained for Cx36-EGFP, E8Q, and E12Q, respectively, and for E8Q-E12Q mutation gj, ss/gj, init was 0.98 ± 0.08. Number of performed experiments are indicated on the bars; *p < 0.05, ns, non-significant. (D–F) All mutations enhance the uncoupling effect of acidification at 0.01 and 1 mM [Mg2+]p, single E8Q mutation abolish the stimulating effect of acidification at 5 mM [Mg2+]p, while E12Q and double mutation E8Q-E12Q reverses it to a decrease in gj, eff/gj,ss.