Figure 6.

A number of inter-organelle contact sites visualized with the split-GFP system. (A) Diagram of inter-organelle contact sites we tested. The alphabet X in squares corresponds to Fig 6X showing the corresponding inter-organelle contact sites. (B–J) Wild-type cells expressing the indicated split-GFP proteins were imaged by confocal fluorescent microscopy. Su9-RFP, mCherry-PTS1, BipN-mCherry-HDEL and Erg6-mCherry were used to label mitochondria, peroxisomes, the ER and LDs, respectively (organelle marker proteins were summarized in Materials and Methods). The vacuole and LDs were stained with fluorescent dyes, FM4-64 and BODIPY 558/568 C₁₂, respectively. Maximum projection was performed to show split-GFP signals on mitochondria, peroxisomes and LDs while a single focal plane was shown for the ER and vacuole. Dotted lines indicate plasma membranes. Scale bars represent 2 µm.