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. 2018 Apr 18;8:6166. doi: 10.1038/s41598-018-24661-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of Huperzine A and necrostatin-1 on oocyte growth in rhesus macaque antral follicles after 5 weeks of culture in an alginate matrix. Oocytes obtained were surrounded by cumulus cells (A; representative from the vehicle control group). Oocyte growth was determined by measuring oocyte diameters. CC, cumulus cells; CTRL, vehicle control; HupA, Huperzine A addition; Nec1, necrostatin-1 addition. *Significant difference between treatment groups (P < 0.05). Data are presented as the mean ± SEM with 18–31 oocytes per experimental group. Scale bar = 25 µm.