Table 1.
Information about oligonucleotid primers used for RT-PCR.
| Target gene | Direction | Sequence |
Macaca mulatta
fitness |
Accession number |
|---|---|---|---|---|
| MLKL | Forward | 5′- TAC AGT CAG CAG AGT GCA GG -3′ | 85% |
H. sapiens
NM_152649.2 M.Mulatta XM_015126624.1 |
| Reverse | 5′- ACC GTT TGT GGA TGA CCT GG -3′ | 95% | ||
| RIPK1 | Forward | 5′- TGG GCG TCA TCA TAG AGG AAG -3′ | 100% |
H. sapiens
NM_003804 M.Mulatta XM_015135439.1 |
| Reverse | 5′- CGC CTT TTC CAT GTA AGT AGC A -3′ | 100% | ||
| ACHE | Forward | 5′- TTC CTC AGT GAC ACC CCA GA -3′ | 100% |
H. sapiens
NM_000665.4 M. Mulatta NM_001128088.2 |
| Reverse | 5′- GGG GAG AAG AGA GGG GTT AC -3′ | 100% |
Note that primers were designed using Homo sapiens RNA sequences. Except MLKL forward (85%) and MLKL reverse (95%), all primers are 100% identical to Macaca mulatta sequences. Sequences of PCR products obtained matched Macaca mulatta sequences as confirmed upon sequencing.