Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 18;8:6176. doi: 10.1038/s41598-018-24296-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Anti-Plasmodium activity of chromobacteria is restricted to the romidepsin-producing subcluster comprised of C. sp. Panama and C. haemolyticum. (A) Variation in growth of asexual stage P. falciparum NF54 upon incubation with n-butanol extracts of approximately 5 mL of culture supernatants of different Chromobacterium species (LB, 72 h, 30 °C). 0% inhibition is adjusted for parasite growth in vehicle control (1% DMSO) and 100% inhibition is matched to that of 250 nM chloroquine. Results are shown as mean ± standard deviation; significance was determined using a one-tailed one sample t-test to determine whether each treatment significantly lowered parasite growth compared to control (ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001). CQ, chloroquine. CAQU, C. aquaticum; CHAE, C. haemolyticum; CPIS, C. piscinae; CPSE, C. pseudoviolaceum; CSPP, C. sp. Panama; CSUB, C. subtsugae; CVAC, C. vaccinii; CVIO, C. violaceum; CW10, C. haemolyticum W10. (B) UPGMA tree generated from a distance matrix inferred from pairwise genome average nucleotide identity (gANI). A distance of 0.02 indicates that the gANI of two species differs by 2 percent points. Cophenetic Correlation Coefficient (CP) = 0.997961877789881. (C) Alignment between the reference romidepsin biosynthetic gene cluster in C. sp. 968 (C968; GenBank: EF210776.1) to the ones present in the genomes of C. sp. Panama (CSPP) and C. haemolyticum, both MDA0585 (CHAE) and W10 (CW10) strains. Geneious alignment in Geneious v5.4 (global alignment with free end gaps; cost matrix: 65% similarity).