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. 2018 Apr 18;7:67. doi: 10.1038/s41426-018-0070-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Mean virus excretion via the pharynx of the highly pathogenic avian influenza virus A/chicken/Netherlands/emc-3/2014 (H5N8) GsGd clade 2.3.4.4 (group A, Buan-like) of wild ducks, based on virus isolation (a, b) and virus detection by reverse transcription-PCR (RT-PCR) (c, d) of all birds in the group. Eurasian wigeon (EUWI, circle), common pochard (COPO, square), mallard (MALL, triangle pointed up), and common teal (COTE, triangle pointed down). TCID₅₀, median tissue culture infectious dose with minimal detection limit of 0.5 TCID₅₀/ml; Ct cycle threshold. AUC, area under the curve summarizes virus excretion from day 0 to 4 post inoculation (mean ± 95% confidence interval) based on virus isolation (b) and virus detection by RT-PCR (d)