Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 18;9:1537. doi: 10.1038/s41467-018-03904-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Role of H3.3 in various induced cell fate changes. a Schematics of the transdifferentiation process indicating the time-points at which chromatin was collected for preparing H3.3 ChIP-Seq libraries. b Venn diagram revealing the number of uniquely and commonly bound genes among day 0 H3.3, day 4 H3.3 and iHP H3.3. c Bar chart revealing the significantly enriched biological processes for genes which are bound by H3.3 on day 4 and in iHP (dark grey) and those bound by H3.3 at day 0 and day 4 (orange). X-axis represents the enrichment score of the hypergeometric enrichment test. d Schematics of the knockdown experiment (top). The bar charts below represent the number of cobblestones formed (left) and CFU numbers (right), observed in wells in which the knockdown of the H3.3 had been performed. Non-targeting siRNA constructs (siNT) were used as controls. The images above the bar charts are representative images of the wells in which the counting took place. Scale bars equal 100 µm. Values are mean ± s.e.m from independent replicate experiments (n = 3). Two-tailed t-test was used for statistical analysis. Error bars represent standard deviation. e Bar chart demonstrating the level of expression of the indicated genes at day 1 and day 2 of the induced neuronal differentiation after the knockdown of H3.3 (siH3.3). Non-targeting siRNA (siNT) were used as controls. Y-axis represents the fold change over control sample. *p-value <0.05. Two-tailed t-test was used for statistical analysis. Error bars represent standard deviation (n = 3). f Bar chart demonstrating the level of expression of the indicated genes at day 4 and day 6 of the induced differentiation to neuronal lineage after the knockdown of H3.3 (siH3.3). Non-targeting siRNA (siNT) were used as controls. Y-axis represents the fold change over control sample. *p-value <0.05. Two-tailed t-test was used for statistical analysis. Error bars represent standard deviation (n = 3)