Figure 1.

Rescue mutant viruses and Identification of gJ expression. (A) Purification and enrichment of mutant viruses. Purification and enrichment of mutant viruses were obtained by the three times passage after transfection. (B) Immunofluorescence detection of gJ expression. DEF cells were infected at 1000 TCID50, and gJ expression was detected by indirect immunofluorescence at 36 hpi. Rabbit anti-gJ were used as primary antibody, and goat anti-rabbit IgG TRITC were used as secondary antibody. (C) Anti-gJ monoclonal antibody (MAb) was used to detect gJ via western immunoblot analysis. DPV CHv-BAC-ΔgJ infected DEF cells were detected as parental virus.