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. 2018 Apr 18;9(5):444. doi: 10.1038/s41419-018-0473-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b The immunocytochemistry, c qPCR analysis and d western blot results revealed that the expression levels of REST decreased in the siREST-treated RPC cultures compared with the control. e The proliferation ability of the RPCs was assessed via CCK-8 analysis. The proliferation ability of the RPCs markedly decreased after treatment with siREST under proliferation conditions. f According to the qPCR analysis, expression levels of Ki-67 decreased significantly in siREST-treated RPC cultures. g-i Immunocytochemistry with antibodies against BrdU and Ki-67 revealed the effects of siREST on RPC proliferation and was consistent with the results shown above. Scale bars: 100 μm. Data are the averages of three independent experiments. Error bars indicate the standard error of the mean. *P ≤ 0.01, **P ≤ 0.01, ***P ≤ 0.001 (Student’s t-test)