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. 2018 Apr 18;9(5):444. doi: 10.1038/s41419-018-0473-5

Table 1.

Primers used for qPCR

Gene Accession no. Forward (5′-3′) Reverse (5′-3′) Annealing temperature [°C] Product size (base pairs)
Ki-67 X82786 cagtactcggaatgcagcaa cagtcttcaggggctctgtc 60 170
Nestin NM_016701 aactggcacctcaagatgt tcaagggtattaggcaagggg 60 235
Pax-6 NM_013627 agtgaatgggcggagttatg acttggacgggaactgacac 60 269
β3-Tublin NM_023279 cgagacctactgcatcgaca cattgagctgaccagggaat 60 152
Rhodopsin NM_145383 tcaccaccaccctctacaca tgatccaggtgaagaccaca 60 216
Recoverin NM_009038 atggggaatagcaagagcgg gagtccgggaaaaacttggaata 60 184
Brn3a NM_0111434 cgctctcgcacaacaacatga ttcttctcgccgccgttga 60 121
Calbindin NM_009788 ggcttcatttcgacgctgac acgtgagccaactctacaattc 60 184
REST NM_005612 gtgcgaactcacacaggaga aagaggtttaggcccgttgt 60 201
Casepase-3 NM_004346 catggaagcgaatcaatggact ctgtaccagaccgagatgtca 60 139
β-Actin NM_007393 agccatgtacgtagccatcc ctctcagctgtggtggtgaa 60 152