Fig. 2. DEPTOR deletion in proximal tubules ameliorated cisplatin-induced AKI.

a IHC staining of DEPTOR in control and DEPTOR cKO mice after treatment with saline or cisplatin. b PAS staining of kidney tissues from control and DEPTOR cKO mice after treatment with saline or cisplatin. c Injury scores for PAS-stained kidney sections. n = 6. d qPCR analysis of Kim1 mRNA levels. e qPCR analysis of NGAL mRNA levels. f BUN and (g) serum creatinine levels, and (h) urinary protein/creatinine ratio in the paired mice. BUN, creatinine and urinary protein were measured with the IDEXX Catalyst Chemistry Analyzer. n = 6–11. All samples were from the mice on day 3 after cisplatin treatment. n = 6. Bars indicate mean values ± SEM. *P < 0.05; **P < 0.01. Scale bar = 20 μm. KIM1 kidney injury molecule 1, NGAL neutrophil gelatinase-associated lipocalin; both are markers for acute renal tubule injury