Fig. 3. Cell apoptosis and cell proliferation in the kidneys of control and DEPTOR cKO mice before and after cisplatin-induced AKI.
a Cell apoptosis analysis with a fluorometric TUNEL assay (green). Nuclei were stained with DAPI (blue). b Quantitative data for TUNEL-positive cells. Data are expressed as the number of TUNEL-positive signals per field (×400). c Cell proliferation analysis with Ki67 immunofluorescence (green). d Quantitative data for Ki67-positive cells. Data are expressed as the number of Ki67-positive signals per field (×400). All samples were from the mice on day 3 after cisplatin treatment. Scale bar = 20 μm. Bars indicate mean values ± SEM. n = 6. **P < 0.01