Fig. 2. CDK4 expression levels correlate to clinicopathological characteristics of synovial sarcoma patients.

CDK4 levels in human synovial sarcoma tissue microarray were determined by immunohistochemistry, and the correlation of CDK4 expression with clinicopathological characteristics of synovial sarcoma patients was evaluated. a Distribution of CDK4 staining scores among clinical stage≥IIB and clinical stage<IIB synovial sarcoma tissues. b Distribution of CDK4 staining scores in the synovial sarcoma tissues from TNM grade≥G2 patients and TNM grade<G2 patients. c Representative images of different immunohistochemical staining intensities of CDK4 ( × 200; scale bar=100 µm). On the basis of the percentage of cells with positive nuclear staining, CDK4 staining patterns were categorized into 6 groups: 0, no nuclear staining; 1+:<10% of positive cells; 2+, 10–25% of positive cells; 3+, 26–50% of positive cells; 4+, 51–75% of positive cells; 5+,>75% of positive cells