Fig. 6. Inhibition of CDK4/6-Rb pathway by palbociclib alters phosphorylation of Rb and promotes apoptosis in human synovial sarcomas.

a Human synovial sarcoma SYO-1 and Fuji cells were treated with increasing concentrations of palbociclib for the indicated time, and the morphologic changes of cells were observed by microscopy after 2 days of palbociclib treatment (scale bar, 50 μm). The expression of respective proteins in CDK4/6-Rb pathway and apoptosis in SYO-1 (b) and Fuji (c) cells was examined by western blotting after 3 days of palbociclib treatment