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. 2018 Apr 18;9:1546. doi: 10.1038/s41467-018-03947-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Site-directed mutational analysis of the interaction between LGI1 and ADAM22 or ADAM23. Pull-down assay between LGI1 mutants and ADAM22 ECD or ADAM23 ECD. Indicated LGI1 mutants and ADAM22 ECD-FLAG or ADAM23 ECD-FLAG secreted from HEK293T cells were mixed and pulled-down with anti-FLAG antibody agarose. Shown are Western blots of the (co-)purified (upper two panels) and input (bottom) samples with indicated antibodies (a). Quantification of the amounts of the co-purified LGI1 with ADAM22 ECD-FLAG (orange) or with ADAM23 ECD-FLAG (light blue) is shown in the graph (b). Results are shown as mean ± s.e. (n = 4 independent experiments)