Figure 2.

Wogonin induces concentration-dependent apoptosis in EoL-1 cells but not in THP-1, PRMI2650, and HMC-1 cells. (A) Representative western blots of HIF-1α, and cleaved caspase-3 in EoL-1, THP-1, RPMI 2650, and HMC-1 cells after wogonin treatment. Cells were treated with wogonin and incubated under normoxic (21% O₂) or hypoxic (1% O₂) conditions for 8 hours. Apoptosis was observed by detecting caspase-3 cleavage (17 kDa) using western blotting. (B) Cells were treated with 100 μM wogonin and incubated under normoxic or hypoxic conditions. Cells were stained with annexin-V and propidium iodide (Lou, #17) and subjected to flow cytometric analyses. (C) The number of cells in the above histograms were counted and plotted as bar graphs. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01. Mann-Whitney U test.