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. 2018 Apr 18;8:6201. doi: 10.1038/s41598-018-24356-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of HIF-1α down-regulates survivin expression in EoL-1 cells. (A) Cells were treated with wogonin and incubated under normoxic or hypoxic conditions for 4 hours. HIF-1α, PARP, caspase-3 and survivin protein expression was detected. Survivin expression was up-regulated in EoL-1 cells under hypoxia. Wogonin decreases HIF-1α and down-regulates survivin expression in EoL-1 cells. (B) Survivin overexpression in EoL-1 cells delays cell apoptosis after wogonin treatment (C) EoL-1 and THP-1 cells transfected with 60 nM HIF-1α siRNA (si-HIF1α) or scrambled siRNA (Si-Cont) were incubated under normoxia or hypoxia for 8 hours. Cells were treated with 100 μM wogonin in hypoxic conditions. Cell lysates were prepared for immunoblotting with the indicated antibodies. (D,E) EoL-1 and THP-1 cells stained with annexin-V and propidium iodide were subjected to flow cytometry. Cells were treated with 100 μM wogonin.