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. 2018 Apr 18;9:1522. doi: 10.1038/s41467-018-03884-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N. benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N. benthamiana. d BiFC assays show that BAK1 interacts with AGG3 in N. benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N. benthamiana. e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP;35S:Myc-AGB1, or pBRI1:BRI1-GFP;35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP;35S:GFP or pBRI1:BRI1-GFP;35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP;35S:BAK1-HA and 35S:GFP-AGG3;35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c, d