Fig. 7.

BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis, respectively. The wild type, bri1-301, and bak1-4 leaf protoplasts co-transformed with 35S:AGB1-Myc and 35S:GPA1-HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b–d Quantification of relative interactions between GPA1 and AGB1 in wild type (b), bri1-301 (c) and bak1-4 (d) leaf protoplasts treated without or with 2% glucose as shown in a. The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD (n = 3) relative to the value for 0% glucose, set at 1. Values in b–d are given as mean ± SD, **P < 0.01 compared with the value for 0% glucose treatment by Student’s t-test