Fig. 5.

SLIGRL-induced conducted vasodilation along the feed artery of mesenteric artery bifurcations isolated from WT and db/db mice. Schematic (A) and confocal fluorescence micrograph (B) of experimental setup and delivery of 10 μM SLIGRL (green) to the downstream end of the artery via the bifurcation. The artery is aligned with sites (distance, mm) for diameter values used to calculate percentage vasodilation in (C) and (D). Arteries from db/db mice were incubated with L-NAME and 45 mM K⁺. Some arteries from WT mice were also perfused with 40 mM glucose or 29 mM mannitol prior to SLIGRL perfusion. Data are expressed as the peak (C) and normalized (D) responses. By normalizing data to 50% dilation at 0 mm, more subtle differences related to the decay of dilation with distance from the bifurcation were observed, n = 5–11. # denotes statistical significance compared to WT, db/db, and db/db + L-NAME. * denotes statistical significance compared to WT control. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)