Figure 5.

Electrophoretic mobility shift assay (EMSA) showing the association of SlHY5 with promoters of SGA biosynthetic genes SlGAME1, SlGAME4, and SlGAME17. (A) SDS–PAGE gel stained with Coomassie blue showing affinity purification of the recombinant SlHY5 protein used for the EMSA. (B) SlHY5 binds directly to the G-box (CACGTG) element of the promoters of SlGAME1, SlGAME4, and SlGAME17. Biotin-labeled DNA probes for the promoters or mutated probes were incubated with GST-HY5 protein and the DNA-protein complexes were separated on 6% native polyacrylamide gels. + or ++ indicate increasing amounts of unlabeled probes as the competitor.