Figure 6.

Electrophoretic mobility shift assay (EMSA) showing the association of SlPIF3 with promoters of SGA biosynthetic genes SlGAME1, SlGAME4, and SlGAME17. (A) SDS–PAGE gel stained with Coomassie blue showing affinity purification of the recombinant SlPIF3 protein used for the EMSA. (B) SlPIF3 binds directly to the promoters of SlGAME1, SlGAME4, and SlGAME17 containing G-box (CACGTG) elements. Biotin-labeled DNA probe from the promoters or mutant probes were incubated with GST-PIF3 protein and the DNA-protein complexes were separated on 6% native polyacrylamide gels. + or ++ indicate increasing amounts of unlabeled probes as the competitor.