Figure 7.

Chromatin immunoprecipitation reveals direct binding of HY5 and PIF3 to the promoters of genes involved in SGA metabolism. (A) Western blot to determine the quality of the polyclonal antibodies against plant proteins. – indicates negative serum; + indicates polyclonal anti-PIF3 and anti-HY5 respectively. (B) Agarose gel electrophoresis analysis of bound sequences through quantitative real-time PCR.

1. Chromatin extract precipitated without antibody (mock).
2. Chromatin extract (input).
3. ChIP with antibodies (anti-HY5 and anti-PIF3, respectively). Analyzed target genes are listed to the left of the corresponding images.

(C) The input percentages derived for different antibodies. The columns represent the average input percentage from three independent experiments. (D) Quantification of ChIP analysis corresponding to images presented in (C). Ordinates show the fold difference.