FIG. 5.
Cq values as a function of years at −80°C values for genes with different turnover rates. Gene expression was determined with samples with RIN values kept constant at 8.5 for all samples. This RIN value was selected because samples with an RIN of 7.5 could not be found at each of the years in storage. Each point represents one sample with the indicated RIN values measured in triplicate for gene expression. Cq values were determined for genes with reported different half lives based on transcription inhibition studies34, (A) 4 hours, (B) 8 hours, (C) stable.