Table 3.

Genes Tested by Polymerase Chain Reaction

Gene symbol	Gene name	RNA half-life	RNA (ng)	Biological function	Assay IDa
PAK2	p21 Protein-activated kinase 2	4 Hours	80	Protein amino acid phosphorylation	Hs02559219_s1
SERBP1	Serpine mRNA binding protein 1	4 Hours	40	Regulation of mRNA stability	Hs00967385_g1
TUBA4A	Tublin, alpha 4a	4 Hours	40	Mitotic cell cycle	Hs01081794_g1
ACO1	Aconitase 1, soluble	8 Hours	60	Tricarboxylic acid cycle	Hs00158095_m1
NAPA	N-ethylmaleimide-sensitive factor attachment protein, alpha	8 Hours	40	Intracellular protein function, brain development	Hs00943303_m1
PRDX5	Peroxiredoxin 5	8 Hours	40	Inflammatory response to oxidative stress	Hs00738905_g1
ETFB	Electron-transfer flavoprotein, beta polypeptide	Stable	40	Respiratory electron transport chain	Hs01085511_m1
GSTM5	Glutathione S-transferase, mu5	Stable	80	Glutathione and xenobiotic metabolism	Hs00757076_m1
MCTS1	Malignant T cell-amplified sequence 1	Stable	60	Regulation of transcription	Hs01585687_m1
ACTB	Beta-actin	Stable	40	Protein folding	Hs01060665_g1
				Cytoskeletal protein

TaqMan Gene Expression Assays (FAM-dye/MBG probe) examined in this study. The RNA half-life listed in the table was determined in fibroblasts and B cells by chemically stopping degradation, which may not reflect the values in postmortem brain tissue.³⁰ The amount of RNA listed corresponds to the quantity used to form cDNA for determination of Cq value of each analyzed gene.

^aPurchased from Applied Biosystems.

cDNA, complementary DNA; Cq, cycle of quantification; mRNA, messenger RNA.