Fig. 2.

Vps33b and Vipar deficient mice have defects in terminal keratinocyte differentiation. A - K14 staining in control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 skin sections, counterstained with DAPI. Scale bars = 20 μm. Images are a maximum projection of a z-stack and are representative of results from at least six control and three Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 independent murine biopsies. B - TEM images of control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 SC. Scale bars = 200 nm. Images are representative of at least two independent murine biopsies per genotype. C - Quantification of corneocyte height: the two corneocytes closest to the SG-SC junction were measured. D - Quantification of cornified envelope thickness. SB, SG and SC layers are indicated. ** p ≤ 0.01, *** p ≤ 0.001, ****p ≤ 0.0001.