Fig. 5.

Vps33b and Vipar knockout affects collagen I deposition in fibroblasts and fibroblast function. A - Decellularised matrix from primary murine fibroblast cultures stained for collagen I. Scale bars = 200 μm. Images are maximum projections of a z-stack. Three images of two independent matrices analysed per genotype. B – Standard deviation (SD) of the Gaussian function of Directionality analysis of fibres in “A”. C - Range (Max - Min) of the Gaussian function of Directionality analysis of fibres in ‘A’. ns p > 0.05, * p ≤ 0.05, **** p ≤ 0.0001. D - Full thickness skin models of control or Vipas39^fl/flER^T2 primary fibroblasts with control or Vps33b^fl/flER^T2 primary keratinocytes stained with H&E, and anti-KLK5. H&E scale bar = 20 μm, KLK5 scale bar = 50 μm. Images are representative of at least three full thickness models.